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Image Search Results
Journal: Journal of Clinical Investigation
Article Title: Loss of acinar cell IKKα triggers spontaneous pancreatitis in mice
doi: 10.1172/jci64498
Figure Lengend Snippet: Figure 2 Loss of pancreatic IKKα causes acinar cell death and compensa- tory proliferation. (A) Amylase and lipase activities in sera of 6- or 12-week-old IkkαF/F and IkkαΔpan mice. (B) Trypsin activity in pan- creata of 2- or 5-month-old IkkαF/F and IkkαΔpan mice. (C) TUNEL assay and apoptotic cell index. Original magnification, ×200. (D and E) H&E staining of IkkαΔpan pancreatic sections showing aci- nar cell apoptosis (D) and necrosis (E). Original magnification, ×400. (F) BrdU incorporation and label- ing index in pancreata of 3-month- old IkkαF/F and IkkαΔpan mice. Original magnification, ×400. (G) Ki67 staining and labeling index of pancreatic sections from 3-month- old mice. Original magnifica- tion, ×400. Results are shown as mean ± SEM. n = 4–6 mice per condition. **P < 0.01. Scale bars: 50 μm; 10 μm (insets).
Article Snippet: IB, IHC, and IF were conducted with antibodies to the following: IKKα (Imgenex), LC3B (Cell Signaling), p62 (Santa Cruz Biotechnology Inc. and Promgen), ERK (Cell Signaling), HA (Roche), Flag (Sigma-Aldrich), ATG16L2 (Abgent), Ub (Santa Cruz Biotechnology Inc.), LAMP1 and LAMP2 (Santa Cruz Biotechnology Inc.),
Techniques: Activity Assay, TUNEL Assay, Staining, BrdU Incorporation Assay, Labeling
Journal: Oncology letters
Article Title: Solamargine triggers hepatoma cell death through apoptosis.
doi: 10.3892/ol.2015.3194
Figure Lengend Snippet: Figure 6. (A) Expression of apoptosis‑associated proteins in SMMC7721 and HepG2 cells treated with SM and (B) the statistical results of this analysis. The expression levels of Bcl‑2, Bax, caspase‑3 and caspase‑9 were detected by western blot analysis using β‑actin as a control. (C) Expression levels of proliferation‑associated proteins, ki67 and pcna, also investigated by western blot analysis and (D) the statistical results of this analysis. The results are representative of at least three independent experiments. SM, solamargine.
Article Snippet:
Techniques: Expressing, Western Blot, Control